We are glad to announce the publication of “Fish Parasites: A Handbook of Protocols for their Isolation, Culture and Transmission”, the first book of the EAFP Book series under an agreement with 5M publishing.
The prevailing situation in aquatic animal health demands a better understanding of fish parasitic diseases and therefore, more control over these. However, this is a challenging task, one that starts with three simple, yet sophisticated, steps: the isolation of the agent, the in vitro culture, and the experimental transmission of the parasite.
This book offers step-by-step procedures for isolating these parasites and reproducing parasitic infections under laboratory conditions. It covers different endo and ectoparasites from diverse taxonomic groups, both protozoans and metazoans (ciliates, flagellates, amoebae, myxozoans, helminths, oomycetes and crustaceans). Most of the parasites we describe can be transmitted directly from fish to fish, with no need for intermediates or vectors, but others require the precise intervention of several hosts. For some parasites in vitro culture has been achieved, for others in vivo continuous passage in fish is needed. With our book, we wanted readers to find information quickly and easily, so for each parasite chapter we have dedicated a short background information including the life cycle and the disease invoked and then details on the facilities, equipment and reagents needed and standard operating procedures with summary templates. The target audience for this volume comprises not only scholars and students, but also researchers wanting to tackle fish parasites and are reluctant to do so, due to the inherent initial difficulties.
The work performed along five years within ParaFishControl, a research project funded by the European Union Horizon 2020 framework, marked the inspirational inception of this handbook. European specialists in fish parasitology conceived the content of this book, but it soon broadened and grew with international collaborators. This book, indeed, represents the interdisciplinary, broad nature of Parasitology, a science that gathers the expertise of veterinarians, biologists, pharmacists, chemists, epidemiologists, and many more.
Enjoy reading and practising the protocols!